追踪食品中的沙门氏菌和李斯特菌快速检测和菌株鉴别

Rapid DetectionChasing Salmonellaand Listeriain Food: Rapid detection and strain discriminationOlaf Degen, Director Industry MicrobioogyBruker 09 May 2024||© 2023 BrukerBRUKER MICROBIOLOGY & INFECTION DIAGNOSTICS22SalmonellaCronobacterColiformsCampylobacterProteomicsTechnology: MALDI-TOF MS -youcanutilizeitfor…Food & Industrial Microbiology13/05/2024L. monocytogenesLactic acid bacteriaYeasts & MoldsFilamentous Fungi…confirmation of: …identification of: Degen, Food Safety Summit 2024||© 2023 BrukerXLD AgarGRAM-NEGATIVEFOOD PATHOGENSDAY 1Liquid enrichmentDAY 2Transfer to selectiveagars: Streakon XLD & ChromogenicAgar -Incubation: 37.0°C, 24hDAY 3PresumptiveSalmonella, needsconfirmationSAVE workload and Time-to-resultISO 6579: Salmonella ScreeningISO-confirmationConfirmationbyMALDI Biotyper®DAY 4 & 5(normal workflow):Confirmation includes plating on non-selective Agar plates for 24h and biochemical tests. In many cases followed by Salmonellaserology (subtyping).33Qualitycontrol of ingr