葡萄球菌肠毒素B诱导的急性呼吸窘迫综合征中巨噬细胞的异质性

Heterogeneity of macrophages in Staphylococcal Enterotoxin B induced Acute Respiratory Distress SyndromeKiesha Wilson, PhDAssistant ProfessorDepartment of Pathology, Microbiology, & ImmunologyAcute Respiratory Distress Syndrome (ARDS)•Acute Respiratory Distress Syndrome (ARDS) •Cytokine storm•Fluid build•Epidemiology•30-40% mortality based on severity of disease.•No Cure•Treatment includes: Ventilation, Oxygen Support, and Fluid Management. Matthay et al (2019)Staphylococcus Enterotoxin B (SEB) •Staphylococcal Enterotoxin B (SEB)•Superantigenic region of SEB similar to SARS-CoV-2 spike protein motif (Function Differs)•Superantigen sAGbinds to MHC and activates T-cells•Toxic Shock Syndrome, Food Poisoning, ARDS, Sepsis•BiowarfareCheng et al (2021)SuperantigenConventionalAntigenAntigen Binding CleftPROJECT OVERVIEW•SEB-induced ARDS is fatal in C3H/HeJmice when given as a dual dose within 7 days•Tissues collected 72 hours post-toxin introduction•Single Cell RNA-seq and Single Cell ATAC-se